# **Primer3-py** documentation

**Primer3-py** is a Python-abstracted API for the popular Primer3 library. The
intention is to provide a simple and reliable interface for automated oligo
analysis and design.

Routine oligo analysis is extremely simple:

```
>>> import primer3
>>> primer3.calc_tm('GTAAAACGACGGCCAGT')
49.16808228911765
>>> primer3.calc_hairpin('CCCCCATCCGATCAGGGGG')
ThermoResult(structure_found=True, tm=34.15, dg=337.09, dh=-36300.00, ds=-118.13)
```

... and `fast` (**1000X** faster than traditional subprocess wrappers):

```
In [1]: import primer3

In [2]: import tests.wrapper

In [3]: %timeit primer3.calc_tm('GTAAAACGACGGCCAGT')
100000 loops, best of 3: 4.74 µs per loop

In [4]: %timeit tests.wrappers.calc_tm('GTAAAACGACGGCCAGT')
100000 loops, best of 3: 5.78 ms per loop
```

**Primer3-py** also includes bindings for the Primer3 `primer design engine`
if you'd prefer to use an established pipeline. The IO parameters mirror those
of the original Primer3, but you don't have to deal with messy and slow file
IO for your automated workflows.

**Please note that while we provide bindings, we do not provide support for
the Primer3 design engine. Please contact the Primer3 dev team with your
questions: https://github.com/primer3-org/primer3 **

A copy of the Primer3 2.6.1 design parameters manual can be found at:
[primer3 v2.6.1 manual](https://htmlpreview.github.io/?https://github.com/primer3-org/primer3/blob/v2.6.1/src/primer3_manual.htm)

## Contents

```{toctree}
:maxdepth: 2

quickstart
api/index
cython_help
miscellany
changes_link
```

## Indices and tables

- {ref}`genindex`
- {ref}`modindex`
- {ref}`search`